A. Animal Use: The animals authorized for use in this protocol will be used only in the activities and in the manner described herein, unless a modification is specifically approved by the CMU ACUC prior to its implementation.

B. Duplication of Effort: I have made every effort to ensure that this protocol is not an unnecessary duplication of previous experiments.

C. Statistical Assurance: I assure that I have consulted with a qualified individual who evaluated the experimental design with respect to the statistical analysis, and that the minimum number of animals needed for scientific validity will be used.

D. Biohazard/Safety: I have taken into consideration and made the proper coordination regarding all applicable rules and regulations concerning radiation protection, biosafety, recombinant issues, and so forth, in the preparation of this protocol.

E. Training: I verify that the personnel performing the animal procedures or manipulations described in this protocol are technically competent and have been properly trained to ensure that no unnecessary pain or distress will be caused to the animals because of the procedures or manipulations.

F. Responsibility: I acknowledge the inherent moral, ethical and administrative obligations associated with the performance of this animal use protocol, and I assure that all individuals associated with this project will demonstrate a concern for the health, comfort, welfare, and well-being of the research animals.

G. Scientific Review: This proposed animal use protocol has received appropriate peer scientific review and is consistent with good scientific research practice.

H. Painful Procedures: I am NOT conducting biomedical experiments, which may potentially cause more than momentary or slight pain or distress to animals.

I. Research studies: CMU ACUC will be notified of any changes in the proposed project, or personnel, relative to this application. I will not proceed with animal experiment until approval by the CMU ACUC is granted.